《植物生理学报》 2016, 52(3): 317-324

基于转录组和蛋白质组关联研究技术筛选紫花苜蓿耐盐相关候选基因

张振亚, 裴翠明, 马进^*

浙江农林大学风景园林与建筑学院, 浙江临安311300

通信作者：马；E-mail: majinzjl@163.com

摘　要：

通过高通量多组学数据的整合分析, 可以为诠释植物耐盐分子机制提供新思路。以南方型紫花苜蓿‘Millennium’为材料, 对正常培养和盐胁迫条件下的2个样品根系进行转录组和蛋白质组关联分析, 以期发现紫花苜蓿耐盐相关候选基因。结果表明: 蛋白质组和转录组相关性并不高, 定量蛋白和基因关联系数为−0.0013; 变化趋势相反差异蛋白和基因的表达关联系数为−0.3648; 变化趋势相同差异蛋白和基因的表达关联系数为0.2620。鉴定出与差异蛋白表达趋势相同的差异基因25个, 上调表达14个, 下调表达11个, 这些差异基因涉及代谢、信号转导、蛋白质降解、防御、抗氧化、细胞骨架、膜与转运、转录和其他未知等方面的功能。另外, 关联分析发现TIP1;1型水通道蛋白(AQP)、钙调素结合蛋白(CaMBP)、富亮氨酸重复类受体激酶(LRR-RLK)、C2H2型锌指蛋白(ZFP)、β-1,3-葡聚糖酶(β-1,3-Glu)、晚期胚胎富集蛋白(LEA)、蔗糖合成酶(SS)、ATP结合盒转运蛋白(ABC)和III类过氧化物酶(PRXs)等差异基因可能在紫花苜蓿耐盐中发挥重要作用。该研究表明: 利用转录组和蛋白质组的关联研究技术可以有效地发现紫花苜蓿耐盐相关候选基因, 为阐明紫花苜蓿对盐胁迫的应答机制提供新线索。

关键词：南方型紫花苜蓿; 盐胁迫; 蛋白质组; 转录组; 关联

收稿：2015-11-23   修定：2016-02-04

资助：国家自然科学基金(31272494)、浙江省自然科学基金(LY16C170003)和浙江省林学重中之重一级学科研究生创新项目(201533)。

Screening of candidate salt tolerance–related genes in alfalfa based on transcriptome–proteome correlation research techniques

ZHANG Zhen-Ya, PEI Cui-Ming, MA Jin^*

Faculty of Landscape Architecture, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang 311300, China

Corresponding author: MA Jin; E-mail: majinzjl@163.com

Abstract:

Integrative analysis of high-throughput multi-omics data would provide innovative perspectives on the molecular mechanisms of plant adaptation to salt stress. Correlation analysis of transcriptomic and proteomic was performed in two southern type alfalfa (Medicago sativa ‘Millenium’) samples of control and Na-Cl-treated samples in order to find salt tolerance–related candidate genes in alfalfa. Moderate correlations between transcriptome and proteome levels were found in this study. Correlation between gene expression and that of quantified protein was −0.0013, as results of a correlation of 0.2620 between genes and proteins with a similar expression trend and that of −0.3648 between those showed opposite trends. Tewnty-five differentially expressed genes showed the same trend as proteins, among which 14 were up-regulated and 11 were down-regulated. These differentially expressed genes were involved in various biological processes such as metabolism, signal transduction, posttranslational modification, protein turnover, chaperones, defense mechanisms, antioxidant, cytoskeleton, transcription and function unknown. In addition, association analysis indicates that differentially expressed genes such as TIP1;1 type aquaporin (AQP), calmodulin-binding protein (CaMBP), leucine-rich repeat receptor-like kinase (LRR-RLK), C2H2 type zinc finger protein (ZFP), β-1,3-glucanase (β-1,3-Glu), late embryogenesis abundant protein (LEA), sucrose synthase (SS), ABC transporter family protein, class III peroxidases (PRXs), etc., played an important role in alfalfa response to salt stress. The research indicates that transcriptome/ proteome-associated research technique was effective to find salt tolerance–related candidate genes in order to provide a new clue for further investigation of the salt tolerance mechanisms in alfalfa.

Key words: southern type alfalfa; salt stress; proteome; transcriptome; correlation